What is the patern of pcDNA3.1?

The pcDNA3.1 plasmid has the following pattern:

* Multiple cloning site (MCS): This region contains a series of restriction enzyme sites that allow you to insert your gene of interest into the plasmid.

* T7 promoter: This promoter is located upstream of the MCS and is used to drive expression of your gene of interest.

* BGH polyadenylation signal: This signal is located downstream of the MCS and is used to terminate transcription of your gene of interest and add a poly(A) tail to the mRNA.

* Ampicillin resistance gene: This gene confers resistance to the antibiotic ampicillin, allowing you to select for bacteria that have successfully taken up the plasmid.

* ColE1 origin of replication: This region is essential for the replication of the plasmid in bacteria.

* Kanamycin resistance gene: This gene confers resistance to the antibiotic kanamycin, allowing you to select for bacteria that have successfully taken up the plasmid.

The pcDNA3.1 plasmid is a versatile expression vector that can be used to express a wide variety of genes in mammalian cells.