Surgical Handwashing: Step-by-Step Protocol for Infection Prevention

Surgical hand antisepsis is a standardized, multi-step process to reduce transient and resident flora on hands/forearms before sterile procedures.

Typical Protocol (WHO / AORN aligned)

  1. Pre-wash: Remove jewelry; trim nails; wet hands/forearms.
  2. Antiseptic application: Apply approved agent (chlorhexidine gluconate 2–4% or povidone-iodine).
  3. Systematic scrubbing (2–6 minutes per agent guidelines):
    • Palm-to-palm
    • Backs of hands / interdigital
    • Fingernails / fingertips (against palm)
    • Thumbs (rotational)
    • Wrists and forearms (to 2 inches above elbow)
  4. Rinse: Fingertips to elbows, avoid splashing.
  5. Dry: Sterile towel, aseptic technique.
  6. Don sterile gloves.

Key Principles

  • Keep hands above elbows during rinse/dry.
  • Do not touch non-sterile surfaces after scrub.
  • Follow facility-specific agent and time requirements.

Consistent adherence reduces surgical site infections (SSIs) and protects patients.

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